helper virus

Recombinant AAV was obtained from the cell culture supernatant after adding helper virus .

重组质粒转染BHK细胞，加入辅助病毒后，得到表达目的蛋白的重组AAV。

Helper virus and virus infectious clone .

对辅助病毒和缺陷病毒进行了传染性克隆。

Detection of helper virus gene in tnf - α gene-transduced DNL of oral carcinoma

转导TNF-α基因的口腔癌DNL中辅助病毒基因的检测

Effects of an induced satellite RNA of cucumber mosaic virus on the host reactions of its helper virus and virus loading in systemic hosts

添加卫星RNA对黄瓜花叶病毒寄主反应及其在系统寄主中含量的影响

Sat-RNA of CMV is a subviral agent , which depends on its helper virus to replicate and cell to cell diffuse .

CMV的卫星RNA是一种依赖其辅助病毒CMV进行复制和扩散的亚病毒因子。

The amphotropic viruses in supernatants were titrated to 1 3 × 10 5CFU / ml. No helper virus could be found by both nested PCR and rescue assay .

双嗜型病毒生产细胞的滴度为13×105CFU/ml，巢式PCR和补救分析均未检测到辅助病毒存在；

Successful integration of Neo R gene into human leukemic cells ( HL-60 , K562 , and KG-1 ) was verified by polymerase chain reaction ( PCR ) . No helper virus could be found by both nested PCR and rescue assay .

重组病毒转导白血病细胞（HL－60，K562，KG－1）后，聚合酶链反应（PCR）证实NeoR基因整合到靶细胞基因组，巢式PCR与补救分析均未能检测到辅助病毒。

By double stranded RNA analysis and sequence determination , the additional satellite RNA in the new strain was found co-existent continuously with genomic RNAs of the helper virus , and no mutation occurred during the pseudo-recombination and co-translation onto different host plants .

通过寄主反应测定、双链RNA分析和全序列分析，显示satYi与CMV-CNa基因组RNA能够稳定共存，经过反复转接也没有发现卫星RNA的序列突变。

To this end , the design of the dozens of pairs of amplification primers for different proto-oncogene J subsets of avian leukemia virus chimeric molecules to the gag gene and the fps gene chimera molecule , and further amplified by the helper virus and defective virus wholly genome .

为此，设计了数十对针对不同原癌基因与J亚群禽白血病毒嵌合体分子的引物扩增到了gag基因与fps基因的嵌合体分子，并进一步扩增了辅助病毒和缺陷病毒的全基因组。